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anticancer monoterpene indole alkaloids in Catharanthus roseus. RNA in situ hybridization has proven a key Pioneer work in 1999 discovered the involvement of epidermis and laticifer idioblasts in the intermediate involved in this pathway. We introduce here a comparison of advantages and drawbacks of in situ hybridization RNA In Situ Hybridization of Paraffin Sections to Characterize the Multicellular Compartmentation of block organization of the pathway. Since then RNA in situ hybridization was routinely used to map the In this Chapter of the "Catharanthus roseus, Methods and Protocols" book, we provide a step-by-step protocol plant tissue array preparation, prehybridization, in situ hybridization, stringent washing and immunodetection RNA In Situ Hybridization of Paraffin Sections to Characterize the Multicellular Compartmentation of

Identifying Major Drivers of Antioxidant Activities in Complex Polyphenol Mixtures from Grape Canes. Molecules considering possible interactions that may occur in complex polyphenol mixture. Using UPLC-MS-based metabolomics classification we explored the polyphenol variations in grape cane extracts from a collection of European In this article published in Molecules, we identified the main drivers of antioxidant activities in polyphenol-rich polyphenol-rich grape cane extracts in European varieties. Congrats to our PhD student Manon Ferrier Identifying Major Drivers of Antioxidant Activities in Complex Polyphenol Mixtures from Grape Canes

minor genome highlights conserved evolutionary traits in monoterpene indole alkaloid synthesis . G3 Bethesda These genes were functionally annotated and used in a comparative genomic analysis to establish gene Dr Vincent Courdavault email vincent.courdavault@univ-tours.fr minor genome finally assembled! Our new publication in G3! The Vinca minor plant synthesizes monoterpene minor genome highlights conserved evolutionary traits in monoterpene indole alkaloid synthesis

"Picturing Empires: Photography and social change in 19th century multi-ethnic Environnments" "The beginning of industrial photography in France : Which representations of the working environment

and halogenated plant monoterpene indole alkaloids in yeast. Nat Chem Biol. 2023 Nov 6. doi 10.1038 s41589-023-01430-2 biosynthesis of two MIAs serpentine and alstonine in baker's yeast Saccharomyces cerevisiae and deploy heterologous expression of a modified halogenase in the microbial MIA platform we document de novo halogenation Dr Vincent Courdavault email vincent.courdavault@univ-tours.fr Published in Nature Chemical Biology, this collaborative work demonstrates a scalable de novo manufacturing metabolites that possess various medicinal properties) in yeast and provides a foundation for further pathway and halogenated plant monoterpene indole alkaloids in yeast

prenylated proteins in signaling. Here we show through biochemical molecular and in planta approaches that growth development and defense. However their role in specialized metabolism is poorly understood. Previously biosynthesis of monoterpene indole alkaloids MIA in Madagascar periwinkle Catharanthus roseus indicating Additionally their transcripts showed higher expression in most parts than other CrROPs. Protein-protein interaction whereas CrROP2 was localized to the plasma membrane. In planta functional studies revealed that silencing Dr Vincent Courdavault email vincent.courdavault@univ-tours.fr geranylgeranylation motif modulate alkaloid biosynthesis in Catharanthus roseus

play a significant role in coping with a wide range of environmental cues in both prokaryotic and eukaryotic proteins were hitherto investigated in a limited number of fungal species. In this study we took advantage of Structure and distribution of sensor histidine kinases in the fungal kingdom. Curr Genet. 2024 Sep 14 70 1 nineteen HK groups have been previously described in the fungal kingdom. However the structure and distribution of recent genomic resources in fungi to refine the fungal HK classification by deciphering the structural Dr Vincent Courdavault email vincent.courdavault@univ-tours.fr Structure and distribution of sensor histidine kinases in the fungal kingdom

trends in the development of metabolomics tools and their potential to address new issues in natural Abstract The increasing demand for natural ingredients in cosmetics raises new challenges for the characterization of standardized extracts. Metabolomics has spread in a variety of research areas with great success for The current sources of natural ingredients used in skincare products are summarized. The actual metabolomics protection and the brightening attributes inherent in natural extracts. Finally we presented the contributions The increasing demand of natural ingredients in cosmetics raises new challenges for the characterization Metabolomics applications in natural cosmetics: addressing the new challenges of bio-sourced ingredients

metabolism in medicinal plant biotechnology through plant microbiome interactions Current Opinion in Plant alternatives such as in vitro plant cultures offer promising solutions. Nonetheless SM production in these systems microbial coculture in a controlled environment. The successful co-culture of in vitro medicinal plants production of pharmaceutically important SM. Keywords In vitro cultures Medicinal plants Specialized metabolites In this article we propose expanding the concept of culturomics to encompass the co-culture of synthetic synthetic microbial communities with medicinal plant in vitro culture. This approach will take advantage Unlocking specialized metabolism in medicinal plant biotechnology through plant-microbiome interactions

Advances in decomposing complex metabolite mixtures using substructure- and network-based computational class information thereby supporting pivotal tasks in metabolomics analysis including metabolite annotation comparison of metabolic profiles and network analyses. In this review we highlight and explain key tools and of these tools aim at processing and analyzing liquid chromatography coupled to mass spectrometry fragmentation as natural products discovery pharmacokinetic and drug metabolism studies and environmental metabolomics Advances in decomposing complex metabolite mixtures using substructure- and network-based computational