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challenging. In particular little is known about the subcellular localization of NMTs in MIA-producing diseases. It is established that MIAs are produced in planta due to complex biosynthetic pathways engaging also a complex tissue and subcellular organization. In this context N-methyltransferases NMTs represent unprecedented data regarding the targeting of these enzymes in planta our results point out an additional layer A research article published in Plant and Cell Physiology in november 2021. Dr Vincent Courdavault email vincent.courdavault@univ-tours.fr γ-tocopherol N-methyltransferase Homologs Involved in the Synthesis of Monoterpene Indole Alkaloids

will investigate the significance of multilingualism in two countries Canada France and the development of of multilingual repertoires for student teachers in relation to their investments and processes of social are how and why are the student teachers engaged in French How do they envision teaching and learning societal multilingualism and multilingual repertoires in French language pedagogy and official curriculum multilingual student teachers of French as a second language in Ontario : from volition to professionnal insertion

to guide their own endeavors in the characterization of missing steps in plant metabolic pathways. Keywords Indole Alkaloid Biosynthetic Pathway Elucidation in Catharanthus roseus from Amor Stander E Dugé de Bernonville next-generation sequencing NGS technologies have resulted in an explosion of available RNA-seq data igniting a In this Chapter of the "Catharanthus roseus, Methods and Protocols" book, we describe bioinformatic protocols Indole Alkaloid Biosynthetic Pathway Elucidation in Catharanthus roseus

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synthesized in planta from tryptamine. In this experiment a potential novel dimeric MIA was discovered in leaf still unknown in which tissues Rauvolfia produce reserpine and where the individual steps in the biosynthetic DESI-MSI. In stem tissue reserpine and many of the intermediates were found compartmentalized in the xylem proposed intermediates were detected in the normal version as well as in the isotope labelled versions confirming comprehensive spatial mapping of metabolites in the R. tetraphylla plant. In addition the article also contains In this study published in Phytochemistry, we explore how matrix assisted laser desorption ionization (DESI) mass spectrometry imaging (MSI) can be used in the investigation of a proposed biosynthetic pathway Spatial localization of monoterpenoid indole alkaloids in Rauvolfia tetraphylla by high resolution mass spectrometry

insights into the regulation of specialized metabolism in plants. Nat Plants. 2022 . doi 10.1038 s41477-022-01288-7 network. Specialized metabolites have important roles in development and adaptation to external cues and they We also discuss how this new knowledge could help in improving strategies to produce useful plant-derived Dr Vincent Courdavault email vincent.courdavault@univ-tours.fr Published in Nature Plants, this review focuses on translational, transcriptional, epigenetic and chromatin-based and also discuss how this new knowledge could help in improving strategies to produce useful plant-derived insights into the regulation of specialized metabolism in plants

Courdavault V. Boosting lignan-precursor synthesis in yeast cell factories through co-factor supply optimization Dr Vincent Courdavault email vincent.courdavault@univ-tours.fr relying on the efficient recycling of enzyme co-factors in yeast to fulfill the synthesis of caffeic acid (CaA) high titer production of valuable natural products in yeast. Boosting lignan-precursor synthesis in yeast cell factories through co-factor supply optimization

anticancer monoterpene indole alkaloids in Catharanthus roseus. RNA in situ hybridization has proven a key Pioneer work in 1999 discovered the involvement of epidermis and laticifer idioblasts in the intermediate involved in this pathway. We introduce here a comparison of advantages and drawbacks of in situ hybridization RNA In Situ Hybridization of Paraffin Sections to Characterize the Multicellular Compartmentation of block organization of the pathway. Since then RNA in situ hybridization was routinely used to map the In this Chapter of the "Catharanthus roseus, Methods and Protocols" book, we provide a step-by-step protocol plant tissue array preparation, prehybridization, in situ hybridization, stringent washing and immunodetection RNA In Situ Hybridization of Paraffin Sections to Characterize the Multicellular Compartmentation of

Identifying Major Drivers of Antioxidant Activities in Complex Polyphenol Mixtures from Grape Canes. Molecules considering possible interactions that may occur in complex polyphenol mixture. Using UPLC-MS-based metabolomics classification we explored the polyphenol variations in grape cane extracts from a collection of European In this article published in Molecules, we identified the main drivers of antioxidant activities in polyphenol-rich polyphenol-rich grape cane extracts in European varieties. Congrats to our PhD student Manon Ferrier Identifying Major Drivers of Antioxidant Activities in Complex Polyphenol Mixtures from Grape Canes

minor genome highlights conserved evolutionary traits in monoterpene indole alkaloid synthesis . G3 Bethesda These genes were functionally annotated and used in a comparative genomic analysis to establish gene Dr Vincent Courdavault email vincent.courdavault@univ-tours.fr minor genome finally assembled! Our new publication in G3! The Vinca minor plant synthesizes monoterpene minor genome highlights conserved evolutionary traits in monoterpene indole alkaloid synthesis